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snap md sox2  (Addgene inc)


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    Structured Review

    Addgene inc snap md sox2
    Snap Md Sox2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/snap+md+sox2/pLV-PGK-SNAP-MD-SOX2+(Plasmid+%23115687)/pmc06890464-264-36-37
    Average 90 stars, based on 1 article reviews
    snap md sox2 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Derivative Assay:

    Article Title: Dynamic regulation of chromatin accessibility by pluripotency transcription factors across the cell cycle
    Article Snippet: 50’000 cells in 1 ml of medium in a 24-well plate were transduced with 50 μl of concentrated lentiviral vector particles to generate stable cell lines. .. pLV-PGK-YPet-MD was derived from pLVTRE3G-Sox2-YPet-MD ( ) by amplification of YPet-MD and restriction cloning into pLV-rtTA3G-IRESBsd using AgeI and SalI. pLV-PGK-SNAP-MD-OCT4 and pLV-PGK-SNAP-MD*-OCT4 were derived by amplification of MD or MD* from SNAP-MD-SOX2 (Addgene #115687) and SNAP-MD*-SOX2 (Addgene #115688) ( ) and restriction cloning into pLVTRE3G-Oct4-YPet ( ) using SalI and XbaI. .. SNAP-MD-OCT4 and SNAP-MD*-OCT4 were further amplified and cloned by restriction cloning into pLV-rtTA3G-IRESBsd ( ) using AgeI and SalI. pLEX-mCherry-OCT4-AID was derived by amplification of OCT4 from pLV-PGK-SNAP-MD-OCT4, AID 71-114 from pEN244 (Addgene #92140) (Nora 2017), and mCherry from pLV-TRE3G-mCherry-PGK-Puro (Suter lab). mCherry and OCT4 were ligated using an XmaI restriction site and mCherry-OCT4 was ligated to AID using a KpnI restriction site.

    Article Title: Dynamic regulation of chromatin accessibility by pluripotency transcription factors across the cell cycle
    Article Snippet: 50’000 cells in 1 ml of medium in a 24-well plate were transduced with 50 μl of concentrated lentiviral vector particles to generate stable cell lines. .. Cloning of overexpression constructs pLV-PGK-YPet-MD was derived from pLVTRE3G-SOX2-YPet-MD ( ) by amplification of YPet-MD and restriction cloning into pLV-rtTA3G-IRESBsd using AgeI and SalI. pLV-PGK-SNAP-MD-OCT4 and pLV-PGK-SNAP-MD*-OCT4 were derived by amplification of MD or MD* from SNAP-MD-SOX2 (Addgene #115687) and SNAP-MD*-SOX2 (Addgene #115688) ( ) and restriction cloning into pLVTRE3G-OCT4-YPet ( ) using SalI and XbaI. .. SNAP-MD-OCT4 and SNAP-MD*-OCT4 were further amplified and cloned by restriction cloning into pLV-rtTA3G-IRESBsd ( ) using AgeI and SalI. pLV-pCAGGS-Tir1-V5 was derived by amplification of pCAGGS-Tir1-V5 from pEN395 (Addgene #92141) ( ) and In-fusion cloning into pLV-PGK-SOX2-SNAP-IRES-Hygro ( ) digested using XhoI and XbaI restriction enzymes. pLEX-mCherry-OCT4-AID was derived by amplification of OCT4 from pLV-PGK-SNAP-MD-OCT4, AID 71–114 from pEN244 (Addgene #92140) , and mCherry from pLV-TRE3G-mCherry-PGK-Puro (Suter lab). mCherry and OCT4 were ligated using an XmaI restriction site and mCherry-OCT4 was cloned into the pLEX_305-C-dTAG backbone (Addgene #91798) ( ) using AgeI and NheI restriction sites.

    Amplification:

    Article Title: Dynamic regulation of chromatin accessibility by pluripotency transcription factors across the cell cycle
    Article Snippet: 50’000 cells in 1 ml of medium in a 24-well plate were transduced with 50 μl of concentrated lentiviral vector particles to generate stable cell lines. .. pLV-PGK-YPet-MD was derived from pLVTRE3G-Sox2-YPet-MD ( ) by amplification of YPet-MD and restriction cloning into pLV-rtTA3G-IRESBsd using AgeI and SalI. pLV-PGK-SNAP-MD-OCT4 and pLV-PGK-SNAP-MD*-OCT4 were derived by amplification of MD or MD* from SNAP-MD-SOX2 (Addgene #115687) and SNAP-MD*-SOX2 (Addgene #115688) ( ) and restriction cloning into pLVTRE3G-Oct4-YPet ( ) using SalI and XbaI. .. SNAP-MD-OCT4 and SNAP-MD*-OCT4 were further amplified and cloned by restriction cloning into pLV-rtTA3G-IRESBsd ( ) using AgeI and SalI. pLEX-mCherry-OCT4-AID was derived by amplification of OCT4 from pLV-PGK-SNAP-MD-OCT4, AID 71-114 from pEN244 (Addgene #92140) (Nora 2017), and mCherry from pLV-TRE3G-mCherry-PGK-Puro (Suter lab). mCherry and OCT4 were ligated using an XmaI restriction site and mCherry-OCT4 was ligated to AID using a KpnI restriction site.

    Article Title: Dynamic regulation of chromatin accessibility by pluripotency transcription factors across the cell cycle
    Article Snippet: 50’000 cells in 1 ml of medium in a 24-well plate were transduced with 50 μl of concentrated lentiviral vector particles to generate stable cell lines. .. Cloning of overexpression constructs pLV-PGK-YPet-MD was derived from pLVTRE3G-SOX2-YPet-MD ( ) by amplification of YPet-MD and restriction cloning into pLV-rtTA3G-IRESBsd using AgeI and SalI. pLV-PGK-SNAP-MD-OCT4 and pLV-PGK-SNAP-MD*-OCT4 were derived by amplification of MD or MD* from SNAP-MD-SOX2 (Addgene #115687) and SNAP-MD*-SOX2 (Addgene #115688) ( ) and restriction cloning into pLVTRE3G-OCT4-YPet ( ) using SalI and XbaI. .. SNAP-MD-OCT4 and SNAP-MD*-OCT4 were further amplified and cloned by restriction cloning into pLV-rtTA3G-IRESBsd ( ) using AgeI and SalI. pLV-pCAGGS-Tir1-V5 was derived by amplification of pCAGGS-Tir1-V5 from pEN395 (Addgene #92141) ( ) and In-fusion cloning into pLV-PGK-SOX2-SNAP-IRES-Hygro ( ) digested using XhoI and XbaI restriction enzymes. pLEX-mCherry-OCT4-AID was derived by amplification of OCT4 from pLV-PGK-SNAP-MD-OCT4, AID 71–114 from pEN244 (Addgene #92140) , and mCherry from pLV-TRE3G-mCherry-PGK-Puro (Suter lab). mCherry and OCT4 were ligated using an XmaI restriction site and mCherry-OCT4 was cloned into the pLEX_305-C-dTAG backbone (Addgene #91798) ( ) using AgeI and NheI restriction sites.

    Cloning:

    Article Title: Dynamic regulation of chromatin accessibility by pluripotency transcription factors across the cell cycle
    Article Snippet: 50’000 cells in 1 ml of medium in a 24-well plate were transduced with 50 μl of concentrated lentiviral vector particles to generate stable cell lines. .. pLV-PGK-YPet-MD was derived from pLVTRE3G-Sox2-YPet-MD ( ) by amplification of YPet-MD and restriction cloning into pLV-rtTA3G-IRESBsd using AgeI and SalI. pLV-PGK-SNAP-MD-OCT4 and pLV-PGK-SNAP-MD*-OCT4 were derived by amplification of MD or MD* from SNAP-MD-SOX2 (Addgene #115687) and SNAP-MD*-SOX2 (Addgene #115688) ( ) and restriction cloning into pLVTRE3G-Oct4-YPet ( ) using SalI and XbaI. .. SNAP-MD-OCT4 and SNAP-MD*-OCT4 were further amplified and cloned by restriction cloning into pLV-rtTA3G-IRESBsd ( ) using AgeI and SalI. pLEX-mCherry-OCT4-AID was derived by amplification of OCT4 from pLV-PGK-SNAP-MD-OCT4, AID 71-114 from pEN244 (Addgene #92140) (Nora 2017), and mCherry from pLV-TRE3G-mCherry-PGK-Puro (Suter lab). mCherry and OCT4 were ligated using an XmaI restriction site and mCherry-OCT4 was ligated to AID using a KpnI restriction site.

    Article Title: Dynamic regulation of chromatin accessibility by pluripotency transcription factors across the cell cycle
    Article Snippet: 50’000 cells in 1 ml of medium in a 24-well plate were transduced with 50 μl of concentrated lentiviral vector particles to generate stable cell lines. .. Cloning of overexpression constructs pLV-PGK-YPet-MD was derived from pLVTRE3G-SOX2-YPet-MD ( ) by amplification of YPet-MD and restriction cloning into pLV-rtTA3G-IRESBsd using AgeI and SalI. pLV-PGK-SNAP-MD-OCT4 and pLV-PGK-SNAP-MD*-OCT4 were derived by amplification of MD or MD* from SNAP-MD-SOX2 (Addgene #115687) and SNAP-MD*-SOX2 (Addgene #115688) ( ) and restriction cloning into pLVTRE3G-OCT4-YPet ( ) using SalI and XbaI. .. SNAP-MD-OCT4 and SNAP-MD*-OCT4 were further amplified and cloned by restriction cloning into pLV-rtTA3G-IRESBsd ( ) using AgeI and SalI. pLV-pCAGGS-Tir1-V5 was derived by amplification of pCAGGS-Tir1-V5 from pEN395 (Addgene #92141) ( ) and In-fusion cloning into pLV-PGK-SOX2-SNAP-IRES-Hygro ( ) digested using XhoI and XbaI restriction enzymes. pLEX-mCherry-OCT4-AID was derived by amplification of OCT4 from pLV-PGK-SNAP-MD-OCT4, AID 71–114 from pEN244 (Addgene #92140) , and mCherry from pLV-TRE3G-mCherry-PGK-Puro (Suter lab). mCherry and OCT4 were ligated using an XmaI restriction site and mCherry-OCT4 was cloned into the pLEX_305-C-dTAG backbone (Addgene #91798) ( ) using AgeI and NheI restriction sites.

    Over Expression:

    Article Title: Dynamic regulation of chromatin accessibility by pluripotency transcription factors across the cell cycle
    Article Snippet: 50’000 cells in 1 ml of medium in a 24-well plate were transduced with 50 μl of concentrated lentiviral vector particles to generate stable cell lines. .. Cloning of overexpression constructs pLV-PGK-YPet-MD was derived from pLVTRE3G-SOX2-YPet-MD ( ) by amplification of YPet-MD and restriction cloning into pLV-rtTA3G-IRESBsd using AgeI and SalI. pLV-PGK-SNAP-MD-OCT4 and pLV-PGK-SNAP-MD*-OCT4 were derived by amplification of MD or MD* from SNAP-MD-SOX2 (Addgene #115687) and SNAP-MD*-SOX2 (Addgene #115688) ( ) and restriction cloning into pLVTRE3G-OCT4-YPet ( ) using SalI and XbaI. .. SNAP-MD-OCT4 and SNAP-MD*-OCT4 were further amplified and cloned by restriction cloning into pLV-rtTA3G-IRESBsd ( ) using AgeI and SalI. pLV-pCAGGS-Tir1-V5 was derived by amplification of pCAGGS-Tir1-V5 from pEN395 (Addgene #92141) ( ) and In-fusion cloning into pLV-PGK-SOX2-SNAP-IRES-Hygro ( ) digested using XhoI and XbaI restriction enzymes. pLEX-mCherry-OCT4-AID was derived by amplification of OCT4 from pLV-PGK-SNAP-MD-OCT4, AID 71–114 from pEN244 (Addgene #92140) , and mCherry from pLV-TRE3G-mCherry-PGK-Puro (Suter lab). mCherry and OCT4 were ligated using an XmaI restriction site and mCherry-OCT4 was cloned into the pLEX_305-C-dTAG backbone (Addgene #91798) ( ) using AgeI and NheI restriction sites.

    Construct:

    Article Title: Dynamic regulation of chromatin accessibility by pluripotency transcription factors across the cell cycle
    Article Snippet: 50’000 cells in 1 ml of medium in a 24-well plate were transduced with 50 μl of concentrated lentiviral vector particles to generate stable cell lines. .. Cloning of overexpression constructs pLV-PGK-YPet-MD was derived from pLVTRE3G-SOX2-YPet-MD ( ) by amplification of YPet-MD and restriction cloning into pLV-rtTA3G-IRESBsd using AgeI and SalI. pLV-PGK-SNAP-MD-OCT4 and pLV-PGK-SNAP-MD*-OCT4 were derived by amplification of MD or MD* from SNAP-MD-SOX2 (Addgene #115687) and SNAP-MD*-SOX2 (Addgene #115688) ( ) and restriction cloning into pLVTRE3G-OCT4-YPet ( ) using SalI and XbaI. .. SNAP-MD-OCT4 and SNAP-MD*-OCT4 were further amplified and cloned by restriction cloning into pLV-rtTA3G-IRESBsd ( ) using AgeI and SalI. pLV-pCAGGS-Tir1-V5 was derived by amplification of pCAGGS-Tir1-V5 from pEN395 (Addgene #92141) ( ) and In-fusion cloning into pLV-PGK-SOX2-SNAP-IRES-Hygro ( ) digested using XhoI and XbaI restriction enzymes. pLEX-mCherry-OCT4-AID was derived by amplification of OCT4 from pLV-PGK-SNAP-MD-OCT4, AID 71–114 from pEN244 (Addgene #92140) , and mCherry from pLV-TRE3G-mCherry-PGK-Puro (Suter lab). mCherry and OCT4 were ligated using an XmaI restriction site and mCherry-OCT4 was cloned into the pLEX_305-C-dTAG backbone (Addgene #91798) ( ) using AgeI and NheI restriction sites.



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